Before running YoYotta check how to Install macFuse on the Mac.
With Big Sur if using an LTO drive with a ATTO SAS interface, then you will need to Install ATTO drivers.
With Big Sur if using an mLogic LTO drive then you will need to Install mTape.
Then run YoYotta and click the at the bottom of the panel to step through the main parts of the interface.
If using an LTO drive or library open the LTFS panel and YoYotta will check and download LTFS.